rhil 21 (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems rhil 21
Rhil 21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil-+21/Recombinant+Human+IL-21+Protein/pmc13089343-228-29-32
Average 94 stars, based on 57 article reviews

rhil 21 - by Bioz Stars, 2026-11

94/100 stars

Images

Isolation:

Article Title: BCMA/CD19 CAR T cell therapy for refractory myasthenia gravis: Proteomic signatures and single-cell transcriptomics of disease flares.
Article Snippet: For AChR- α–specific B cell detection, PBMCs were stained with the CD19 (BioLegend) and AChR- α–loaded dCODE Klickmer and then detected by flow cytometry and laser confocal microscopy (Leica Microsystems). .. B cells (105 per well) isolated from samples collected before CAR T cell therapy and following BCRC were seeded in 96- well plates stimulated with recombinant human CD40L (50 ng/ml; PeproTech), F(ab′)2 fragment goat anti- human IgM (10 μg/ml; Jackson ImmunoResearch) and recombinant human IL- 21 (50 ng/ml; R&D Systems) for 7 days. .. Then, B cells were collected for antibody cocktail staining in response to ASCs differentiation [anti–CD19allophycocyanin (APC), anti–CD27- BV421, anti- CD38–fluorescein isothiocyanate (FITC) and anti- CD138- APC- Cy7] (all from BioLegend) as described above.

Article Title: Organ injury in systemic autoimmunity is mediated by stem-like CD8 + T cells arising from tissue-draining lymph nodes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PE anti-mouse IL-21R Antibody Biolegend Cat#131906; RRID:AB_1279430 BD PharmingenTM Purified NA/LE Hamster Anti-Mouse CD3e BD Biosciences Cat#553057; RRID:AB_394590 BD PharmingenTM Purified NA/LE Mouse Anti-Mouse CD28 BD Biosciences Cat#566883; RRID:AB_2869934 Ultra-LEAFTM Purified anti-mouse CD8a Antibody Biolegend Cat#100764; RRID:AB_2810324 Ultra-LEAFTM Purified Rat IgG2a, κ Isotype Ctrl Antibody Biolegend Cat#400566; RRID:AB_11148951 TotalSeqTM-C0301 anti-mouse Hashtag 1 Antibody Biolegend Cat#155861; RRID:AB_2800693 TotalSeqTM-C0302 anti-mouse Hashtag 2 Antibody Biolegend Cat#155863; RRID:AB_2800694 TotalSeqTM-C0303 anti-mouse Hashtag 3 Antibody Biolegend Cat#155865; RRID:AB_2800695 InVivoMAb rat IgG2a isotype control, antitrinitrophenol BioXCell Cat#BE0089; RRID:AB_1107769 InVivoMAb anti-mouse CD122 (IL-2Rβ) BioXCell Cat#BE0272; RRID:AB_2687795 InVivoMAb anti-mouse IL-21R BioXCell Cat#BE0258; RRID:AB_2687737 InVivoMAb anti-mouse PD-1 (CD279) BioXCell Cat#BE0273; RRID:AB_2687796 CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscienceTM Invitrogen Cat#16-0037-85; RRID:AB_468855 CD28 Monoclonal Antibody (CD28.2), Functional Grade, eBioscienceTM Invitrogen Cat#16-0289-85; RRID:AB_468927 anti-Nephrin guinea pig polyclonal, serum PROGEN Cat#GP-N2; RRID:AB_2904121 Complement C3 Polyclonal Antibody Invitrogen Cat#PA5-21349; RRID:AB_11153785 APC Goat anti-mouse IgG (minimal x-reactivity) Antibody Biolegend Cat#405308; RRID:AB_315011 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat#ab150185; RRID:AB_2736871 Goat Anti-Mouse IgG, Human ads-AP SouthernBiotech Cat#1030-04; RRID:AB_2794293 Biological samples Lupus patient PBMCs Yale University N/A Chemicals, peptides, and recombinant proteins LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit Invitrogen Cat#L34966 Recombinant Mouse IFN-gamma Protein with carrier R&D Systems Cat#485-MI-100 Recombinant Mouse TNF-alpha (aa 80-235) Protein with carrier R&D Systems Cat#410-MT-025 Recombinant Mouse IL-10 Protein with carrier R&D Systems Cat#417-ML-025 Recombinant Mouse IL-12 Protein with carrier R&D Systems Cat#419-ML-010 Recombinant Mouse IL-15 Protein with carrier R&D Systems Ca#447-ML-010 Recombinant Mouse IL-18/IL-1F4 Protein with carrier R&D Systems Cat#9139-IL-050 Recombinant Mouse IL-21 Protein with carrier R&D Systems Cat#594-ML-025 Universal Type I IFN Protein R&D Systems Cat#11200-1 Human IL-2 Recombinant Protein PeproTech Cat#200-02 (Continued on next page) ll OPEN ACCESSArticle Immunity 59, 1–21.e1–e15, June 9, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human IL-15 Protein, CF R&D Systems Cat#BT-015-010 Recombinant Human IL-21 Protein with carrier R&D Systems Cat#8879-IL-010 FTY720 MilliporeSigma Cat344597; CAS:162359-56-0 Deoxyribonucleic acid from calf thymus MilliporeSigma Cat#D4522; CAS:91080-16-9 Reacti-Bind TM DNA Coating Solution Thermo Fisher Cat#17250 DAPI Nucleic Acid Stains Invitrogen Cat#D1306 ProLongTM Gold Antifade Mountant Invitrogen Cat#P36930 Collagenase from Clostridium histolyticum MilliporeSigma Cat#C9263; CAS: 9001-12-1 Renal Epithelial Cell Basal Medium ATCC Cat#PCS-400-030 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10x Genomics Cat#CG000365 Chromium GEM-X Single Cell 5’ Kit 10x Genomics Cat#CG000733 EasySep Mouse CD4+ TCell Isolation Kit STEMCELL Technologies Cat#19852 EasySep Mouse CD8+ TCell Isolation Kit STEMCELL Technologies Cat#19853A EasySepTM Human CD8+ T Cell Isolation Kit STEMCELL Technologies Cat#17953 eBioscienceTM Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Invitrogen Cat#00-5521-00 PierceTM Bradford Protein Assay Kit Thermo Scientific Cat#23200 QuantiChromTM Creatinine Assay Kit BioAssay Systems Cat#DICT-500 BD PharmingenTM PE Annexin V Apoptosis Detection Kit I BD Biosciences Cat#559763; RRID:AB_2869265 Deposited data Mouse single cell sequencing data This study Gene Expression Omnibus (GEO) accession number: GSE318514 Human single cell sequencing data Accelerating Medicines Partnership Phase II RA/SLE AMP2 Lupus Kidney Single Cell Mouse strains MRL/MpJ-Faslpr/J The Jackson Laboratory 000485; RRID:IMSR_JAX:000485 NZBWF1/J The Jackson Laboratory 100008; RRID:IMSR_JAX:100008 B6.Cg-Sle1NZM2410/Aeg Yaa/DcrJ The Jackson Laboratory 021569; RRID:IMSR_JAX:021569 B6N.B6-Tg(Nr4a1-EGFP/cre)820Khog/J The Jackson Laboratory 018974; RRID:IMSR_JAX:018974 C57BL/6J The Jackson Laboratory 000664; RRID:IMSR_JAX:000664 MRL/MpJ-Faslpr/J × B6N.B6-Tg(Nr4a1EGFP/cre)820Khog This study N/A MRL/MpJ-Faslpr/J.TCRβ-/- Peng et al.53 10.1084/jem.184.3.1149 Software and algorithms FlowJo v10.8.1 TreeStar FlowJo LLC. .. RRID:SCR_008520 GraphPad Prism v9.5.1 GraphPad Software RRID:SCR_002798 ImageJ imagej.net/ij RRID:SCR_003070 CellRanger v5.0.1 10xgenomics.com/support/software/ cell-ranger RRID:SCR_023221 R version 4.2.0 r-project.org RRID:SCR_001905 chromVAR v1.18 bioconductor.org/packages/chromVAR RRID:SCR_026570 circlize v0.4.16 cran.r-project.org/web/packages/circlize RRID:SCR_002141 cisTopic v0.3.0 github.com/aertslab/cisTopic N/A clusterProfiler v4.6.2 bioconductor.org/packages/clusterProfiler RRID:SCR_016884 Clustree v0.5.0 cran.r-project.org/web/packages/ clustree/index RRID:SCR_016293 (Continued on next page) ll OPEN ACCESS Article e3 Immunity 59, 1–21.e1–e15, June 9, 2026

Article Title: BCMA/CD19 CAR T cell therapy for refractory myasthenia gravis: Proteomic signatures and single-cell transcriptomics of disease flares
Article Snippet: For AChR-α–specific B cell detection, PBMCs were stained with the CD19 (BioLegend) and AChR-α–loaded dCODE Klickmer and then detected by flow cytometry and laser confocal microscopy (Leica Microsystems). .. B cells (10 5 per well) isolated from samples collected before CAR T cell therapy and following BCRC were seeded in 96-well plates stimulated with recombinant human CD40L (50 ng/ml; PeproTech), F(ab′)2 fragment goat anti-human IgM (10 μg/ml; Jackson ImmunoResearch) and recombinant human IL-21 (50 ng/ml; R&D Systems) for 7 days. .. Then, B cells were collected for antibody cocktail staining in response to ASCs differentiation [anti–CD19-allophycocyanin (APC), anti–CD27-BV421, anti-CD38–fluorescein isothiocyanate (FITC) and anti-CD138-APC-Cy7] (all from BioLegend) as described above.

Recombinant:

Article Title: A novel method for generating chimeric antigen receptor-transduced primary human natural killer cells by the use of multiple cytokines and anti-CD2 and anti-NKp46 stimulatory antibodies.
Article Snippet: PBMCs containing 60,000 NK (CD56+, CD3-) cells were seeded in 8 mL of CellGenix GMP SCGM (CellGenix GmbH, Freiburg, Germany) supplemented with 10 % FBS. .. The following cytokines were added at the following concentrations: recombinant human IL-2 (27 ng/mL) (Peprotech, Cranbury, NJ, USA), recombinant human IL-12 (10 ng/mL) (R&D Systems, MN, USA), recombinant human IL-18 (10 ng/mL) (R&D Systems, MN, USA), recombinant human IL-21 (10 ng/mL) (R&D Systems, MN, USA). .. The PBMCs were cultured with dissolvable hydrogel microspheres harboring anti-CD2 and anti-NKp46 antibodies (Cloudz Human NK Cell Expansion Kit, Bio-Techne, MN, USA) in a T-25 flask at 37◦C and 5 % CO2 in an incubator according to the manufacture’s instruction with slight modifications.

Article Title: A Feeder Cell-Free System for Chimeric Antigen Receptor Gene Transduction Into Natural Killer Cells
Article Snippet: Recombinant human IL-18 (R&D Systems, catalog number: 9124-IL) 10. .. Recombinant human IL-21 (R&D Systems, catalog number: 8879-IL) 11. .. Polybrene solution (Nacalai Tesque, catalog number: 12996-81) 12.

Immunopeptidomics:

Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination
Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, rhIL-21 (30 ng/ml; R&D Systems, 8879-IL) for 3 days in 48-well plate. rhIL-2 (30 IU/ml; R&D Systems, 202-IL), rhIL-7 (5 ng/ml; R&D Systems, 207-IL), and rhIL-15 (5 ng/ml; R&D Systems, 247-ILB) were supplemented every 2 days to the cocultured cells for a total duration of 14 days before the expanded T cells were used for immunogenicity validation assays. ..

Biomarker Discovery:

Transfection:

Article Title: Combining computational target prioritization and a B cell maturation assay for target evaluation studies in systemic lupus erythematosus.
Article Snippet: Background and Purpose: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease involving production of autoantibodies by B cells.. This study aimed at identifying novel drug targets using a computational algorithm to select targets and thereafter validate the top ranked 11 targets by siRNA knockdown in a primary B cell maturation assay.. Experimental Approach: The top 1 % genes (~150 genes) from SLE genome-wide association studies were ranked by Priority index (Pi), a computational tool integrating genomic and network information to prioritize diseaserelevant genes.

Suspension:

Concentration Assay:

Article Title: Composition for culturing natural killer cells and method for preparing natural killer cells by using same
Article Snippet: .. TABLE 1 Basal culture medium 1 Components Manufacturer Cat. # Dose Final concentration CTS TM Immune Thermo A2596102 0.5 ml 5% Cell SR (Serum replacement) GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhiL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL AIM-V medium GIBCO 0870112-DK to 50 ml TABLE 2 Basal culture medium 2 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhIL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL AIM-V medium GIBCO 0870112-DK to 50 ml TABLE 3 Basal culture medium 3 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhIL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL X-VIVO TM 15 Lonza 04-418Q to 50 ml Medium TABLE 4 Basal culture medium 4 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% NK MACS Miltenyi Biotec 130-113-102 500 1% supplement anti-CD2/ Miltenyi Biotec 130-094-483 CD335 beads OKT3 Biolegend 317326 10 ng/mL NK MACS 130-112-968 to 50 ml medium TABLE 5 Adding material Classification Components Manufacturer Dose Final concentration Adding GI-101 GI-Innovation adding 1.6 nM/50 nM condition 1 immediately before use Adding GI-101WT GI-Cell adding 1.6 nM/50 nM condition 2 immediately before use Adding CD80-Fc + GI-Cell adding CD80-Fc (1.6 nM) + condition 3 Fc-IL2WT immediately Fc-IL2WT (1.6 nM)/ before use CD80-Fc (50 nM) + Fc-IL2WT (50 nM) Adding CD80-Fc + GI-Cell adding CD80-Fc (1.6 nM) + condition 4 Fc-IL2v2 immediately Fc-IL2v2 (1.6 nM)/ before use CD80-Fc (50 nM) + Fc-IL2v2 (50 nM) ..

Review

rhil 21 (R&D Systems)

Structured Review

Images

Related Articles

Isolation:

Recombinant:

Immunopeptidomics:

Biomarker Discovery:

Transfection:

Suspension:

Concentration Assay:

Single Cell:

Gene Expression:

Cell Isolation:

Bradford Protein Assay:

Sequencing:

Software:

Magnetic Cell Separation:

Similar Products

Image Search Results